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Abstract Trace metals have been an important ingredient for life throughout Earth’s history. Here, we describe the genome-guided cultivation of a member of the elusive archaeal lineage Caldarchaeales (syn. Aigarchaeota ), Wolframiiraptor gerlachensis , and its growth dependence on tungsten. A metagenome-assembled genome (MAG) of W. gerlachensis encodes putative tungsten membrane transport systems, as well as pathways for anaerobic oxidation of sugars probably mediated by tungsten-dependent ferredoxin oxidoreductases that are expressed during growth. Catalyzed reporter deposition-fluorescence in-situ hybridization (CARD-FISH) and nanoscale secondary ion mass spectrometry (nanoSIMS) show that W. gerlachensis preferentially assimilates xylose. Phylogenetic analyses of 78 high-quality Wolframiiraptoraceae MAGs from terrestrial and marine hydrothermal systems suggest that tungsten-associated enzymes were present in the last common ancestor of extant Wolframiiraptoraceae . Our observations imply a crucial role for tungsten-dependent metabolism in the origin and evolution of this lineage, and hint at a relic metabolic dependence on this trace metal in early anaerobic thermophiles. 

Abstract Recent changes in US oceanographic assets are impacting scientists' ability to access seafloor and sub‐seafloor materials and thus constraining progress on science critical for societal needs. Here we identify national infrastructure needs to address critical science questions. This commentary reports on community‐driven discussions that took place during the 3‐dayFUTURE of US Seafloor Sampling Capabilities 2024 Workshop, which used an “all‐hands‐on‐deck” approach to assess seafloor and sub‐seafloor sampling requirements of a broad range of scientific objectives, focusing on capabilities that could be supported through the US Academic Research Fleet (US‐ARF) now or in the near future. Cross‐cutting issues identified included weight and size limitations in the over‐boarding capabilities of the US‐ARF, a need to access material at depths greater than ∼20 m below the seafloor, sampling capabilities at the full range of ocean depths, technologies required for precise navigation‐guided sampling and drilling, resources to capitalize on the research potential of returned materials, and workforce development.